Proteomic Analysis of Jasmonic Acid-Regulated Proteins in Rice Leaf Blades
Abstract:Jasmonates play a critical role in plant defense against pathogens through regulation of the expression of defense- related genes.To study the role of jasmonic acid (JA) in the rice self-defense mechanism, a proteomic approach was applied. When 3-week-old rice cv. Java 14 was treated with 100 μM JA for 3 days, numerous necrotic brown spots were observed on the leaf blade.Three-week-old rice was treated with JA and proteins from cytosolic and membrane fractions of leaf blade were separated by two-dimensional polyacrylamide gel electrophoresis. A total of 305 proteins were detected in both cytosolic and membrane fractions. When rice plant was treated with 100 μM JA for 2 days, 12 proteins were up-regulated and 2 proteins were down-regulated. Out of them, 8 proteins were changed in dose dependence manner, while 4 proteins were changed in a time course manner. Among them, pathogenesis-related protein 5 (PR5) and probenazole inducible protein 1 (PBZ1) were significantly induced by 100 μM JA for 2 days. These results suggest that PR5 and PBZ1 are important proteins expressed down-stream of JA signals in rice cv. Java 14.
Document Type: Research Article
Affiliations: National Institute of Crop Science, Kannondai 2-1-18, Tsukuba 305-8518, Japan.
Publication date: April 1, 2007
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