Cautionary Tail: The Presence of an N-Terminal Tag on Dynein Light-Chain Roadblock/LC7 Affects Its Interaction with a Functional Partner
As part of structural investigations of components of the molecular motor, dynein, we prepared the light chain, Robl1_mouse, with and without an N-terminal His-tag. We found that the His-tag introduced a spurious binding site for a second protein, IC74. We propose a molecular mechanism for functional interference by the His-tag.
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Document Type: Research Article
Affiliations: Department of Biochemistry,University of Wisconsin-Madison, 433 Babcock Drive, Madison WI 3706,USA.
Publication date: 2007-03-01
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