The fast-twitch SERCA1 isoform of the sarcoplasmic reticulum Ca2+-ATPase was purified to homogeneity and conjugated to peroxidase. The SERCA1 probe showed high affinity binding to the immobilized monomeric enzyme, but not crosslinker-stabilized oligomers. This suggests a preferential complex formation via homo-dimerization, rather than interactions with established oligomeric structures.
Department of Biology, National University of Ireland, Maynooth, Co. Kildare, Ireland.
Publication date: March 1, 2007
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