Large Scale Preparation of the Mammalian High Mobility Group Protein A2 for Biophysical Studies
Abstract:Due to asymmetrical charge distribution of the mammalian high mobility group protein A2 (HMGA2), which makes HMGA2 bind to both cation- and anion-exchange columns, we developed a rapid procedure for purifying HMGA2 in the milligram range. This purification procedure greatly facilitated biophysical studies, which require large amounts of the protein.
Keywords: DNA binding protein; The mammalian high mobility group protein A2 (HMGA2); differential scanning calorimetry; intrinsically unstructured protein; isothermal titration calorimetry; nuclear magnetic resonance (NMR) Spectroscopy
Document Type: Research Article
Affiliations: Department of Chemistry and Biochemistry, Florida International University, 11200 SW 8th Street, Miami,Florida 33199, USA.
Publication date: January 1, 2007
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