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Expression of the Canavalia brasiliensis Lectin (ConBr) in Tobacco Plants

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Abstract:

Tobacco plants were transformed with gene constructs encoding prepro-ConBr (Canavalia brasiliensis lectin). Transgenic plants confirmed by PCR expressed the recombinant protein as revealed by Western blot. However, the apparent molecular mass of the recombinant polypeptide (ca. 34 kDa) was higher than the native lectin (about 30 kDa), showing that further proteolytic processing of pro-ConBr was not detected.





Keywords: Canavalia brasiliensis; heterelogous expression; lectin; precursor; processing; recombinant

Document Type: Research Article

DOI: https://doi.org/10.2174/092986606778777434

Affiliations: Laboratorio de Genetica Molecular,Depto. de Biologia, Bloco 906, Campus do Pici, Universidade Federal do Ceara, Av. Humberto Monte s/n, CEP 60.455-970, Fortaleza, Ceara,Brazil.

Publication date: 2006-10-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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