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Two-Dimensional Gel Electrophoresis Based Technologies for Potential Biomarkers Identification in Amniotic Fluid: A Simple Model

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To assess the efficiency of two-dimensional fluorescence difference gel electrophoresis (2D-DIGE) and of twodimensional electrophoresis and ammoniacal silver staining (2D-E), different amounts of soybean trypsin inhibitor and horse myoglobin were added to amniotic fluid. In this model, a minimum of 5 to 10 ng of “artificial” biomarkers was detected.

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Keywords: Amniotic fluid; Biomarker; CyDye; Proteomics; Two-dimensional electrophoresis

Document Type: Research Article

Affiliations: Service regional vaudois de transfusion sanguine, Rue du Bugnon 27, CH-1005 Lausanne, Switzerland.

Publication date: 2006-10-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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