Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA

Authors: Iyaguchi, Daisuke; Yao, Min; Watanabe, Nobuhisa; Nishihira, Jun; Tanaka, Isao

Source: Protein and Peptide Letters, Volume 13, Number 5, May 2006 , pp. 531-533(3)

Publisher: Bentham Science Publishers

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Abstract:

Hepatocyte nuclear factor 6 (HNF-6)/OC-1, a part of liver-enriched transcription factor, controls pancreas and liver development and regulates expression of several hepatic genes. DNA-binding region of HNF-6α bound to a 14-mer DNA fragment has been crystallized by the hanging drop vapor diffusion method. The crystals belong to space group P2 with unit cell parameters of a = 73.0 Å, b = 39.0 Å, c = 106.5 Å, β= 107.6 . X-ray diffraction data were collected to 2.0 Å resolution.

Keywords: HNF-6; Transcription regulator; Homeobox; ONECUT; Crystallization

Document Type: Research article

DOI: http://dx.doi.org/10.2174/092986606776819592

Affiliations: 1: Division of Biological Sciences, Graduate School of Science, Hokkaido University, Sapporo, Japan.

Publication date: 2006-05-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.