Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA
Authors: Iyaguchi, Daisuke; Yao, Min; Watanabe, Nobuhisa; Nishihira, Jun; Tanaka, Isao
Source: Protein and Peptide Letters, Volume 13, Number 5, May 2006 , pp. 531-533(3)
Publisher: Bentham Science Publishers
Abstract:Hepatocyte nuclear factor 6 (HNF-6)/OC-1, a part of liver-enriched transcription factor, controls pancreas and liver development and regulates expression of several hepatic genes. DNA-binding region of HNF-6α bound to a 14-mer DNA fragment has been crystallized by the hanging drop vapor diffusion method. The crystals belong to space group P2 with unit cell parameters of a = 73.0 Å, b = 39.0 Å, c = 106.5 Å, β= 107.6 . X-ray diffraction data were collected to 2.0 Å resolution.
Document Type: Research article
Publication date: 2006-05-01
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