Structure-Based Stabilization of an Enzyme: The Case of Penicillin Acylase from Alcaligenes faecalis
Abstract:The modeled structure of penicillin acylase from Alcaligenes faecali (AFPGA) was constructed by comparative modeling with the Modeller program. Candidate positions that could be replaced with cysteine were estimated by scanning the modeled structure of AFPGA with the program MODIP (modeling disulfide bond in protein). The mutant Q3C/P751C had A higher optimum temperature by three degrees than that of the wild type AFPGA. The half life of the double mutant Q3C/P751C at 55°C was increased by 50%. To our knowledge, this was the first structure-based genetic modification of AFPGA.
Document Type: Research Article
Affiliations: State Key Laboratory of Bioreactor Engineering, New World Institute of Biotechnology, East China University of Science and Technology, Shanghai 200237, P.R. China.
Publication date: February 1, 2006
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