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A Nonradioactive Assay for Poly(A)-Specific Ribonuclease Activity by Methylene Blue Colorimetry

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A simple nonradioactive assay, which was based on the specific shift of the absorbance maximum of methylene blue induced by its intercalation into poly(A) molecules, was developed for poly(A)-specific ribonuclease (PARN). A good linear relationship was found between the absorbance at 662 nm and the poly(A) concentration. The assay conditions, including the concentration of methylene blue, the incubation temperature and time, and the poly(A) concentration were evaluated and optimized.

Keywords: Enzyme assay; Enzyme inhibitor; Methylene blue colorimetry; Poly(A)-specific ribonuclease

Document Type: Research Article


Affiliations: Life Science Division, Graduate School at Shenzhen, Tsinghua University, Shenzhen 518055, China.

Publication date: 2006-02-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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