Biophysical Characterization of the Non-Fusogenic Interaction Between Liposomes and the Shrimp Bifunctional Lipoprotein-β-Glucan Binding Protein HDL/BGBP
Shrimp High Density Lipoprotein-β-Glucan Binding Protein (HDL/BGBP) has been studied by its role in nutrition and innate defense. Although the mechanisms of lipid loading are still unknown, HDL-BGBP binds and aggregates phospholipids vesicles in vitro. To gain insights into the HDL-BGBP mechanism of interaction with membranes, we have used fluorescence spectroscopy and electron microscopy. Data show that HDL-BGBP does not induce membrane fusion, leakage nor lipid exchange, although microstructural changes are clearly observed. This work supports a model where protein aggregation leads to liposome clustering. Such interaction may be a critical factor for the activation of the shrimp blood cell in vivo.
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Document Type: Research Article
Affiliations: Aquatic Molecular Biology Laboratory, Centro de Investigacion en Alimentacion y Desarrollo, A.C. PO Box 1735, Hermosillo Sonora, 83000 Mexico.
Publication date: 2006-01-01
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