Skip to main content

Expressed Protein Ligation: A New Tool for the Biosynthesis of Cyclic Polypeptides

Buy Article:

$63.00 plus tax (Refund Policy)

Abstract:

The present paper reviews the use of expressed protein ligation for the biosynthesis of backbone cyclized polypeptides. This general method allows the in vivo and in vitro biosynthesis of cyclic polypeptides using recombinant DNA expression techniques. Biosynthetic access to backbone cyclic peptides opens the possibility to generate cell-based combinatorial libraries that can be screened inside living cells for their ability to attenuate or inhibit cellular processes.

Keywords: circular proteins; intein-mediated ligation; native chemical ligation; polypeptide libraries; protein engineering; protein splicing

Document Type: Review Article

DOI: https://doi.org/10.2174/0929866054864274

Affiliations: Chemical Biology and Nuclear Sciences Division, Lawrence Livermore National Laboratory, 7000 East Avenue, Livermore, CA 94550;

Publication date: 2005-11-01

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
  • Access Key
  • Free content
  • Partial Free content
  • New content
  • Open access content
  • Partial Open access content
  • Subscribed content
  • Partial Subscribed content
  • Free trial content
Cookie Policy
X
Cookie Policy
Ingenta Connect website makes use of cookies so as to keep track of data that you have filled in. I am Happy with this Find out more