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Expressed Protein Ligation to Study Protein Interactions: Semi-Synthesis of the G-Protein Alpha Subunit

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Interactions between G proteins and GPCRs are fundamental for transmitting signals for a multitude of physiologic responses. Little is known regarding the protein-protein interface between the G protein and the receptor, much less the mechanisms for receptor activation of G proteins. Here, we will describe how expressed protein ligation will aid in the study of protein-protein interactions between semi-synthetic G alpha subunits and GPCRs.

Keywords: expressed protein ligation; g protein; intein; rhodopsin; transducin

Document Type: Review Article


Affiliations: Departments of Medicine, Molecular Biology and Pharmacology, Washington University, St. Louis, MO 63110;

Publication date: 2005-11-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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