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Intein-Mediated, In Vitro and In Vivo Protein Modifications with Small Molecules

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We review intein-mediated approaches for the site-specific modifications of proteins and highlight their applications in (1) the site-specific in vitro and in vivo biotinylation of proteins for protein arrays and (2) the site-specific in vivo labeling of proteins in living cells.

Keywords: bioimaging; biotinylation; intein; native chemical ligation; protein arrays; site-specific

Document Type: Review Article


Affiliations: Department of Chemistry, National University of Singapore, 3, Science Drive 3, Singapore 117543, Republic of Singapore;

Publication date: 2005-11-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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