Mimicking Reverse Protein Splicing by Three-Segment Tandem Peptide Ligation

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Here we report a bi-directional and interchangeable three-segment peptide ligation of N, M, and C-segments, mimicking the reverse process of protein splicing to form, in tandem, a tripartite NMC-peptide using a synthetic intein, a role served by the M-segment with an N-terminal Ser or Thr and a C-terminal thioester.

Keywords: c-to-n tandem ligation; cys ligation; n-to-c tandem ligation; pro ligation; reverse protein splicing; synthetic intein; three-segment tandem ligation; tripartite nmc-protein

Document Type: Review Article

DOI: http://dx.doi.org/10.2174/0929866054864238

Affiliations: Department of Microbiology and Immunology, Vanderbilt University, MCN A5102A, Nashville, TN 37232.

Publication date: November 1, 2005

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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