Crystallization and Preliminary Crystallographic Analysis of Human Eukaryotic Translation Initiation Factor 5A (eIF-5A)
Abstract:Eukaryotic translation initiation factor 5A (eIF-5A) is universally found in all eukaryotic cells. It is the only protein in nature known to contain the unusual amino acid hypusine, a post-translationally modified lysine. Recombinant human eIF-5A was crystallized by the hanging-drop vapor diffusion method. Crystals were grown at 291K using (NH4)2SO4 as precipitant. Diffraction data were obtained to a resolution of 2.7Å from a single frozen crystal belonging to space group C2, with unit-cell parameters a=147.1Å, b=60.4Å, c=76.4Å, β=92.4 . There are more than three molecules per asymmetric unit.
Document Type: Review Article
Affiliations: Laboratory of Structural Biology, Life Sciences Building, Tsinghua University, Beijing, 100084, China;
Publication date: October 1, 2005
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