Protein Expression, Crystallization and Preliminary X-Ray Crystallographic Studies of YjbK from Bacillus subtilis
B. subtilis YjbK is a protein with 190 residues of uncharacterized function, it has been annotated by Pfam database as a member of adenylate cyclase family (EC: 220.127.116.11). In order to identify its exact function via structural studies, yjbK gene was amplified from B. subtilis genomic DNA and cloned into expression vector pET21-DEST. The protein was expressed in a soluble form in E. coli and purified to homogeneity. YjbK was crystallized and diffracted to a resolution of 2.0 Å in-house. The crystals belong to P1 space group, with unit cell parameters a=32.38 Å, b=34.69 Å, c=46.02 Å, α=96.560 , β=99.683° , γ=111.333° . There is one molecule per asymmetric unit.
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Document Type: Review Article
Affiliations: National Laboratory of Protein Engineering and Plant Genetic Engineering, China;
Publication date: 2005-10-01
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