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A Simple Method for Constructing a Tagged Protein

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We have developed a simple method for preparing a tagged protein by PCR. With this method any protein sequence can be easily tagged. The techniques include three steps of DNA restriction, ligation and PCR. We could obtain a DNA construct containing SUMO-1 gene with His6 tag sequence with high efficiency by the next day.
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Keywords: affinity tag; gene fusion; polymerase chain reaction (pcr); recombinant protein; small ubiquitin-related modifier (sumo)

Document Type: Review Article

Affiliations: Department of Biomedical Sciences and Biomedical Brain Research Center, National Institute of Health, 5 Nokbun-dong, Eunpyung-gu, Seoul : 122-701: , Korea;

Publication date: 2005-10-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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