Crystallization of the N-Terminal Ankyrin Repeat Domain of the 2-5ADependent Endoribonuclease, RNase L
The N-terminal ankyrin repeat domain of the 2'-5'-linked oligoadenylate (2-5A)-dependent endoribonuclease, RNase L, has been crystallized by the hanging-drop vapor diffusion method in the presence of 2-5A. The crystals belong to an orthorhombic space group P212121 with cell dimensions of a = 63.11 Å, b = 73.03 Å, and c = 82.64 Å. There is one molecule per asymmetric unit. The crystals diffract to at least 2.1 Åresolution using synchrotron radiation and are suitable for X-ray structure analysis at high resolution.
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Document Type: Review Article
Affiliations: School of Pharmaceutical Sciences, Showa University, Tokyo 142-8555, Japan.
Publication date: 2005-05-01
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