Thermal Inactivation and Unfolding of a Dimeric Arginine Kinase
Abstract:Thermal inactivation and unfolding of the dimeric arginine kinase (AK) from sea cucumber Stichopus japonicus was investigated. The activation energy was calculated to be 388 kJ/mol. Based on the analysis of the denaturation course at 58°C, a model is suggested for the thermal unfolding of this dimeric AK. In addition, the effect of free Mg2+ and the potential biological significance on the thermal unfolding of dimeric AK is discussed.
Document Type: Review Article
Affiliations: Department of Biological Science and Biotechnology, School of Life Sciences and Engineering, Tsinghua University, Beijing 100084 China.
Publication date: May 1, 2005
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