Purification of Cytochrome P450 BM-3 as a Monooxygenase
After investigating two anion-exchange resins, the purification factor and activity yields of P450 BM-3 were higher with Resource Q than with DEAE-Sepharose FF. Screening of HIC media showed that Source 15ISO was the most suitable for purification of P450 BM-3. An effective isolation and purification procedure of P450 BM-3 was developed and included three steps: 35%-70% saturation (NH4)2SO4 precipitation, Source 15ISO hydrophobic interaction chromatograph and Sephacryl S-200 gel filtration chromatography. Using this protocol, the purification factor and P450 BM-3 activity recovery was 13.5 and 13.7%, respectively.
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Document Type: Review Article
Affiliations: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 PR China.
Publication date: 2005-05-01
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