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Affinity Ligands for Industrial Protein Purification

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Abstract:

Significant efforts are put into the design of large-scale purification processes of proteins due to great demands regarding cost efficiency and safety. In order to design an effective purification scheme the unit operations need to be reduced to a minimum. In this review we are discussing proteinaceous ligands as well as small synthetic mimics for use in affinity chromatography for large-scale applications. Different advantages as well as drawbacks of the two approaches are outlined.

Keywords: affinity chromatography; cleaning-in-place (cip); deamidation; protein a; protein engineering; protein g; stabilization

Document Type: Review Article

DOI: http://dx.doi.org/10.2174/0929866053765662

Affiliations: Department of Biotechnology, Royal Institute of Technology (KTH), Albanova University Center, SE-106 91 Stockholm, Sweden.

Publication date: May 1, 2005

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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