Production of Minichaperone (sht GroEL191-345) and Its Function in the Refolding of Recombinant Human Interferon Gamma
Abstract:The recombinant minichaperone sht GroEL191-345 was cultivated in a 3.7 L stirred bioreactor with the high yield of 216.2 mg / L broth. In the refolding of recombinant human interferon gamma (rhuIFN-γ) inclusion bodies, more than 2-3 fold enhancement in protein mass recovery and total activity were observed in the presence of free or immobilized minichaperone to the refolding buffer.
Document Type: Review Article
Affiliations: Department of Chemical and Biochemical Engineering, Zhejiang University, Hangzhou 310027 P. R. China.
Publication date: January 1, 2005
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