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Content of the Hmg-17 Chromosomal Protein in Porcine Tissues

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Abstract:

HMG-17 proteins are nucleosomal proteins, implicated in control of chromatin structure and transcription, in a way that has not yet been fully understood. In this report, quantification of HMG-17 in porcine tissues was performed, by ELISA, using previously produced and characterized specific rabbit anti- HMG-17 antibodies. Our results showed high levels of HMG-17 compared to the DNA and H1 content of the tissues (on the contrary to previous reports), and more specifically that there were 1.7 molecules of HMG-17 per molecule of histone H1.

Keywords: elisa; histone h1; hmg-17; quantification; tissue distribution

Document Type: Review Article

DOI: https://doi.org/10.2174/0929866043406193

Affiliations: Laboratory of Biological Chemistry, and Department of Forensic Medicine and Toxicology, Medical School, University of Ioannina, 451 10 Ioannina, Greece.

Publication date: 2004-12-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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