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Protein Extraction and Comparison of Stain Protocols for Analysis of Two-Dimensional Electrophoresis Gels

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Protein extraction and the proteome of Lactobacillus delbrueckii subsp. bulgaricus were studied using different stains. The reversible silver staining technique was shown to be more sensitive than the irreversible silver stain. Coomassie colloidal was demonstrated to be as sensitive as reversible silver stain; however, the Coomassie colloidal blue solution developed a higher background and for sample preparation was more time-consuming.

Keywords: 2-DE electrophoresis; Lactobacillus bulgaricus; protein extraction; staining

Document Type: Review Article


Affiliations: Escola Superior de Biotecnologia, Universidade Catolica Portuguesa Rua Dr. Antonio Bernardino de Almeida, 4200-072 Porto, Portugal.

Publication date: 2004-04-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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