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Seed Protein Variation Among Pepper (Capsicum Sp.) Genotypes Revealed By Maldi-Tof Analysis

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A method for seed proteome analysis using MALDI-TOF mass spectrometry is described. The data were used to estimate the genetic diversity degree among twelve genotypes of pepper (Capsicum). The resulting spectra were converted into a binary matrix consisting of 23 protein data sets, and genetic similarity values were calculated with the FreeTree software and Jaccard's coefficient of similarity. We have also been able to identify the presence of certain proteins in the extracts, by checking their masses on online databases.
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Keywords: capsicum; genetic variability; germplasm; mass spectrometry; time of flight

Document Type: Review Article

Affiliations: Laboratorio de Citogenetica e Genetica Molecular, Depto. de Biologia, bloco 906, Campus do Pici, Universidade Federal do Ceara (UFC), Av. Humberto Monte s/n, 60.451-970; Fortaleza-CE, Brasil.

Publication date: 2004-02-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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