Salting-In Effects offset Mgcl2-Induced Refolding of Nucleoside Diphosphate Kinase
Previously we reported that halobacterial nucleoside diphosphate kinase can be refolded in the presence of concentrated trimethylamine N-oxide (TMAO) as well as NaCl, indicating that enhancement of compact structure formation by TMAO is sufficient for folding. Here we showed that the refolding effect of MgCl2 is maximal at 1 M and declines to zero at 2 M, indicating that charge shielding effect of MgCl2 is offset by its salting-in effect.
Keywords: charge shielding; halophilic; nucleoside diphosphate kinase; refolding; salting-in
Document Type: Review Article
Affiliations: Faculty of Agriculture, Kagoshima University, 1-21-24 Korimoto, Kagoshima 890-0065, Japan, and Alliance Protein Laboratories, 3957 Corte Cancion, Thousand Oaks, CA 91360, USA.
Publication date: 01 December 2003
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