The Role Of Tyrosine Residues In The Rna N-Glycosidase Activity Of Cinnamomin A-Chain
Abstract:Cinnamomin is a type II ribosome-inactivating protein (RIP) and its A-chain (CTA) is a RNA Nglycosidase. It is observed that modification of tyrosine residues by N-acetylimidazole (N-AI) causes almost complete loss of CTA activity. Adenine partially protects tyrosine residues from modification by NAI. It is proposed that tyrosine residues are involved in the active site of CTA and they are crucial in recognition and binding of ribosomal RNA. Tryptophan residues of CTA are also studied by NBS modification.
Document Type: Review Article
Affiliations: State Key Laboratory of Molecular Biology, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, The Chinese Academy of Science. 320 Yue-yang Road, Shanghai 200031, China.
Publication date: 2003-10-01
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