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Low Content of Protein S29 in Ribosomes of Human Lung Cancer Cell Line A549: Detected by Twodimensional Electrophoresis

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Abstract:



This study revealed that the content of protein S29 in ribosomes of cancer cell line A549 was distinctly low (equivalent to about 30% of that of 2BS). The conclusion was acquired based on the ratios of spot volume of ribosomal protein S29 to that of several other ribosomal proteins (S29 / L37a, S29 / L38, S29 / S27 and S29 / S28) in the same gel plate. The possible biological roles of ribosomal protein S29 in malignant transformation and translation regulation are briefly discussed.





Keywords: 2-dimensional polyacrylamide gel electrophoresis; cbb-chromium modified thiosulfate-silver staining; human liver cancer cell line 7721; human lung cancer; human lung cancer cell line a549; human lung normal cell line 2bs; northern blot; ribosomal protein s29

Document Type: Review Article

DOI: http://dx.doi.org/10.2174/0929866033408273

Publication date: February 1, 2003

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
ben/ppl/2003/00000010/00000001/art00011
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