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Isolation and Characterization of an Angiogeninlike Protein from Goat Plasma

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Abstract:

Angiogenin, a blood vessel inducing protein has been implicated in wound healing and tumour progression. First isolated from human carcinoma cells, it has been subsequently isolated from human, bovine, rabbit, pig and mouse sera and bovine milk. This study reports the isolation of an angiogenic-like protein from goat plasma. The ribonucleolytic activity has been followed by yeast transfer RNA (tRNA) degradation using spectrophotometric and denaturing polyacrylamide gel electrophoresis methods. The chorioallantoic membrane (CAM) assay has been implemented to study its angiogenic activity. The presence of this protein has also been confirmed by strong binding with placental Ribonuclease Inhibitor (PRI).

Keywords: Angiogeninlike Protei; Goat Plasma; Ribonuclease Inhibitor; chorioallantoic membrane

Document Type: Review Article

DOI: http://dx.doi.org/10.2174/0929866023408661

Publication date: August 1, 2002

More about this publication?
  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
ben/ppl/2002/00000009/00000004/art00002
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