Crystallization and Preliminary X-Ray Crystallographic Studies of Trichosanthin Delta C7

Authors: Li X.; Ding Y.; Too H.; Wang Z.; Liu Y.; Dong Y.; Shaw P.; Rao Z.

Source: Protein and Peptide Letters, Volume 9, Number 3, June 2002 , pp. 269-273(5)

Publisher: Bentham Science Publishers

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Abstract:

Trichosanthin (TCS) is a type I ribosome-inactivating protein (RIP) which possesses rRNA N-glycosidase activity. TCS has various pharmacological properties. It is possible to reduce the antigenicity of TCS by deleting up to seven C-terminal residues of TCS (TCS-C7) with minimal effect on its activity [1]. TCS-C7 has been crystallized and the crystal diffracted to 1.8 Å. It belongs to space group P21, with unit-cell parameters a=71.6Å, b=74.4Å, c=87.6Å, bgr =97.0°. It is given that there are four molecules per asymmetric unit.