Purification, Crystallization and Preliminary Crystallographic Studies of a Class Ii Chitinase from Kidney Bean Seeds

Authors: Zhou Z.; Li H.Liu Yikun; Gong W.

Source: Protein and Peptide Letters, Volume 9, Number 3, June 2002 , pp. 265-268(4)

Publisher: Bentham Science Publishers

Buy & download fulltext article:

Price: $63.10 plus tax (Refund Policy)

Abstract:

A new member of class II chitinase from Phaseolus vulgaris was purified and crystallized. Diffraction data to 2.7Å resolution have been collected and the preliminary crystallographic studies have been completed. The space group is P1 with unit cell parameters of a=36.32Å, b=46.24Å, c=70.36Å, agr =97.9 , bgr =103.8 and ggr =110.5 . Molecular replacement and initial refinement statistics indicate there are two chitinase molecules in the crystallographic asymmetric unit.

Keywords: classII chitinase; phaseolus vulgaris chitinase; kidney bean chitinase

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866023408814

Publication date: 2002-06-01

More about this publication?

Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.