Characterization of Pearl Millet Prolamins

Authors: Marcellino L.Helena; Junior C.Bloch; Gander E.Silvano

Source: Protein and Peptide Letters, Volume 9, Number 3, June 2002 , pp. 237-244(8)

Publisher: Bentham Science Publishers

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Abstract:

We report the physical-chemical characterization of the major alcohol-soluble proteins present in seeds of pearl millet (Pennisetum glaucum) by SDS-PAGE, bidimensional gel electrophoresis, MALDI-TOF / MS and RP-HPLC. We demonstrate the presence of three major prolamins, called A-, B- and C-pennisetin with mass values around 27, 22 and 12 kDa, respectively. We present partial amino acid sequences of these major proteins, which should allow the posterior isolation of the respective genes.

Keywords: pearl millet prolamin; pennisetum galaucum; seed storage protein; prolamin; pennisetin

Language: English

Document Type: Review article

DOI: 10.2174/0929866023408724

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