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Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph

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We have characterized the interaction between S-(carbamoylmethyl)-papain and chicken cystatin by means of fluorescence titration at acidic pH. The calculated binding constant was 32 x 10 6, which is less than the tight affinity at pH 7.4 (∼7 x 10 10). Results have been discussed in terms of the burial of Trp-104 of cystatin and the possible role of the charge of the carbamoylmethyl moiety.
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Keywords: carbamoylmethyl-papain; chicken cystatin

Document Type: Review Article

Publication date: 2002-06-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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