Affinity of Chicken Cystatin for S- (Carbamoylmethyl)-Papain, Measured by Fluorescence at Acidic ph

Authors: Arroyo-Reyna A.; Gutierrez-Gomez E.

Source: Protein and Peptide Letters, Volume 9, Number 3, June 2002 , pp. 231-235(5)

Publisher: Bentham Science Publishers

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Abstract:

We have characterized the interaction between S-(carbamoylmethyl)-papain and chicken cystatin by means of fluorescence titration at acidic pH. The calculated binding constant was 32 x 10 6, which is less than the tight affinity at pH 7.4 (sim7 x 10 10). Results have been discussed in terms of the burial of Trp-104 of cystatin and the possible role of the charge of the carbamoylmethyl moiety.

Keywords: carbamoylmethyl-papain; chicken cystatin

Language: English

Document Type: Review article

DOI: 10.2174/0929866023408797

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