The Effect of Fibrillar Aβ1-40 on Membrane Fluidity and Permeability
Abstract:The time course of Aβ fibril formation was characterized using a variety of assays. The effect of Aβ on the membrane fluidity and permeability was assessed by monitoring the anisotropy of the fluorescent probe DPH and TMA-DPH and measuring the release of vesicle-entrapped with three different molecular weight fluorescence probes (ANTS / DPX, Calcein, FD-4). The results show fibrils had formed after 4 days. Aggregated Aβmay decrease the membrane fluidity and induce the leakage of ANTS and Calcein in a dose -dependent manner. These effects may have some relation with Aβ neurotoxicity.
Document Type: Review Article
Affiliations: Department of Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100083 P. R. CHINA
Publication date: 2002-04-01
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