The Effect of Fibrillar A1-40 on Membrane Fluidity and Permeability

Authors: Xiaocui Ma; Yinlin Sha; Kechun Lin; Songqing Nie

Source: Protein and Peptide Letters, Volume 9, Number 2, April 2002 , pp. 173-178(6)

Publisher: Bentham Science Publishers

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Abstract:

The time course of A bgr

fibril formation was characterized using a variety of assays. The effect of A bgr

on the membrane fluidity and permeability was assessed by monitoring the anisotropy of the fluorescent probe DPH and TMA-DPH and measuring the release of vesicle-entrapped with three different molecular weight fluorescence probes (ANTS / DPX, Calcein, FD-4). The results show fibrils had formed after 4 days. Aggregated A bgr

may decrease the membrane fluidity and induce the leakage of ANTS and Calcein in a dose -dependent manner. These effects may have some relation with A bgr

neurotoxicity.

Keywords: fibrillar; membrane fluidity; ants learage; calcein learage

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866023408832

Affiliations: 1: Department of Biophysics, School of Basic Medical Sciences, Peking University, Beijing 100083 P. R. CHINA

Publication date: 2002-04-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.