Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis
A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF / MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.
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Document Type: Review Article
Affiliations: Laboratorio de Bioquimica Marinha - BioMol-Lab, Depto de Engenharia de Pesca, Universidade Federal do Ceara, Caixa postal 6033, CEP 60451-970
Publication date: 2002-04-01
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