Purification and Characterization of a new Lectin from the Red Marine Alga Hypnea Musciformis

Authors: C. S. Nagano1; F.B. M.B. Moreno1; C. Bloch Jr1; M. V. Prates1; J. J. Calvete1; S. Saker-Sampaio1; W.R. L. Farias1; T. D. Tavares1; K. S. Nascimento1; T. B. Grangeiro1; B. S. Cavada1; A. H. Sampaio1

Source: Protein and Peptide Letters, Volume 9, Number 2, April 2002 , pp. 159-165(7)

Publisher: Bentham Science Publishers

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content

Abstract:

A lectin from the red marine alga Hypnea musciformis (HML) was purified by extraction with 20 mM PBS, precipitation with 70% saturated ammonium sulphate, ion-exchange DEAE-Cellulose chromatography and RP-HPLC. The 9.3 kDa polypeptide agglutinates erythrocytes from various sources and shows oligomerization tendencies under certain MALDI-TOF / MS conditions. Preliminary N-terminal sequencing and biological assays strongly suggest that the HML may belong to a new class of algae lectins.

Keywords: hypnea musciformis; red marine alga; lectin

Document Type: Review article

DOI: 10.2174/0929866023408931

Affiliations: 1: Laboratorio de Bioquimica Marinha - BioMol-Lab, Depto de Engenharia de Pesca, Universidade Federal do Ceara, Caixa postal 6033, CEP 60451-970

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$55.10 plus tax      Refund Policy

 

OR

Back to top

Key:
Free Content - Free Content
New Content - New Content
Subscribed Content - Subscribed Content
Free Trial Content - Free Trial Content
Share this item with others: These icons link to social bookmarking sites where readers can share and discover new web pages.
Page Help Click here for Page Help
Shopping cart
Tools
Sign in






Need to register?
Sign up here
Text size: A | A | A | A