Atomic Force Microscopy and Proteins

Author: Silva LP

Source: Protein and Peptide Letters, Volume 9, Number 2, April 2002 , pp. 117-125(9)

Publisher: Bentham Science Publishers

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Abstract:

This review briefly introduces the principles of atomic force microscopy (AFM) applied to protein samples. AFM provides three-dimensional surface images of the proteins with high resolution. The advantage of AFM for protein studies is that AFM can visualize directly the molecule under physiological conditions without previous treatment. AFM operated in the force-spectroscopy mode is now a widespread technique, often used to investigate ligand-receptor interactions with the goal of measuring forces at the individual molecule level.

Keywords: afm; force spectroscopy; proteins adsorption; proteins structure; probe; sample interaction; afm imaging

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866023408869

Affiliations: 1: Departamento de Genetica e Morfologia, UnB, Brasilia, DF, Brazil.

Publication date: 2002-04-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.