Ca(II) -ggr-Thionin complex: Interaction Studies by Differential Pulse Voltammetry and MALDI-TOF / MS

Authors: Pires de Castro C.Silva; DeSouza J.Rodrigues; Junior C.Bloch

Source: Protein and Peptide Letters, Volume 9, Number 1, February 2002 , pp. 45-52(8)

Publisher: Bentham Science Publishers

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Abstract:

In this work we investigated the formation of a complex between ggr-thionin SIagr1 and Ca2+ using differential pulse voltammetry and MALDI-TOF / MS. A stoichiometry rate of one calcium ion per one SIagr1 molecule was obtained. Kd values of 1.31 x 10-9 mol L-1, 2.63 x 10-8 mol L-1 and 2.71 x 10 -8 mol L-1 were determined for the Ca2+-SIagr1 complex by three different methodologies. These findings contribute to a better understanding of the SIagr1 inhibition mechanism towards insect and mammalian agr-amylases.

Keywords: THIONIN COMPLEX; cell-free systems; Matrix-assisted laser; amperometric titration

Language: English

Document Type: Review article

DOI: 10.2174/0929866023409020

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