Ca(II) --Thionin complex: Interaction Studies by Differential Pulse Voltammetry and MALDI-TOF / MS

Authors: Pires de Castro C.Silva; DeSouza J.Rodrigues; Junior C.Bloch

Source: Protein and Peptide Letters, Volume 9, Number 1, February 2002 , pp. 45-52(8)

Publisher: Bentham Science Publishers

Buy & download fulltext article:

Price: $63.10 plus tax (Refund Policy)

Abstract:

In this work we investigated the formation of a complex between ggr -thionin SI agr 1 and Ca2+ using differential pulse voltammetry and MALDI-TOF / MS. A stoichiometry rate of one calcium ion per one SI agr 1 molecule was obtained. Kd values of 1.31 x 10-9 mol L-1, 2.63 x 10-8 mol L-1 and 2.71 x 10 -8 mol L-1 were determined for the Ca2+-SI agr 1 complex by three different methodologies. These findings contribute to a better understanding of the SI agr 1 inhibition mechanism towards insect and mammalian agr -amylases.

Keywords: THIONIN COMPLEX; cell-free systems; Matrix-assisted laser; amperometric titration

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866023409020

Publication date: 2002-02-01

More about this publication?

Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.