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Ca(II) -γ-Thionin complex: Interaction Studies by Differential Pulse Voltammetry and MALDI-TOF / MS

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In this work we investigated the formation of a complex between γ-thionin SIα1 and Ca2+ using differential pulse voltammetry and MALDI-TOF / MS. A stoichiometry rate of one calcium ion per one SIα1 molecule was obtained. Kd values of 1.31 x 10-9 mol L-1, 2.63 x 10-8 mol L-1 and 2.71 x 10 -8 mol L-1 were determined for the Ca2+-SIα1 complex by three different methodologies. These findings contribute to a better understanding of the SIα1 inhibition mechanism towards insect and mammalian α-amylases.
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Keywords: Matrix-assisted laser; THIONIN COMPLEX; amperometric titration; cell-free systems

Document Type: Review Article

Publication date: 2002-02-01

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.
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