Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Authors: Ferreira B.Dos Santos; Branco A.Trindade; de Queiroz K.Salim; de Souza Filho G.Apolinario

Source: Protein and Peptide Letters, Volume 9, Number 1, February 2002 , pp. 39-43(5)

Publisher: Bentham Science Publishers

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Abstract:

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

Keywords: isoelectric protein focusing (IEF); sodium dodecylsulfate (SDS)-PAGE; mercaptoethanol; Ampholytes solution; maize endosperm

Language: English

Document Type: Review article

DOI: 10.2174/0929866023409057

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