Two-dimensional Protein Analysis by Isoelectric Focusing in a Multi-cell Plate System

Authors: Ferreira B.Dos Santos; Branco A.Trindade; de Queiroz K.Salim; de Souza Filho G.Apolinario

Source: Protein and Peptide Letters, Volume 9, Number 1, February 2002 , pp. 39-43(5)

Publisher: Bentham Science Publishers

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Abstract:

A practical and low cost system for isoelectric protein focusing (IEF) was developed. The system uses a multi-cell glass plate compatible with a common vertical one-dimensional electrophoresis chamber, dispensing specific IEF apparatus. After focusing, the IEF gels are easily recovered. The resulting two-dimensional (2-D) gels have provided efficient protein separation for different concentrations and extracts.

Keywords: isoelectric protein focusing (IEF); sodium dodecylsulfate (SDS)-PAGE; mercaptoethanol; Ampholytes solution; maize endosperm

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866023409057

Publication date: 2002-02-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.