Cloning and Expression of Human Beta-defensin-2 Gene in Escherichia Coli

Authors: Fang X.; Peng L.; Xu Z.; Wu J.; Cen P.

Source: Protein and Peptide Letters, Volume 9, Number 1, February 2002 , pp. 31-37(7)

Publisher: Bentham Science Publishers

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Abstract:

Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.

Keywords: Human beta-defensin-2 (hBD-2); proinflammatory agents; anti-Gram-negative; transcription-polymerase chain reaction (RT-PCR); QIAprep Spin Minprep Kit; Chelating column; Coomassie brilliant blue G-250

Language: English

Document Type: Review article

DOI: 10.2174/0929866023409011

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