Cloning and Expression of Human Beta-defensin-2 Gene in Escherichia Coli

Authors: Fang X.; Peng L.; Xu Z.; Wu J.; Cen P.

Source: Protein and Peptide Letters, Volume 9, Number 1, February 2002 , pp. 31-37(7)

Publisher: Bentham Science Publishers

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Abstract:

Human beta-defensin-2 (hBD-2), a small cationic peptide, exhibits a broad range of antimicrobial activity. It has been found to play important roles in innate and adaptive immune responses against microbial invasion. For the purposes of this study, hBD-2 gene was cloned from the lesions of human condyloma acuminatum. An expression vector was constructed and transformed into E. coli. hBD-2 was expressed as a fusion protein in both the soluble and insoluble forms, which was further confirmed by western blotting analysis.

Keywords: Human beta-defensin-2 (hBD-2); proinflammatory agents; anti-Gram-negative; transcription-polymerase chain reaction (RT-PCR); QIAprep Spin Minprep Kit; Chelating column; Coomassie brilliant blue G-250

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866023409011

Publication date: 2002-02-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.