Crystallization And Preliminary X-Ray Studies Of A Thermostable Dna Photolyase From Thermus Thermophilus Hb8

Authors: Komori H.; Tsujiuchi H.; Masuii R.; Kuramitsu s.; Yokoyama S.; Shibata T.; Inoue Y.; Miki K.

Source: Protein and Peptide Letters, Volume 8, Number 6, December 2001 , pp. 495-498(4)

Publisher: Bentham Science Publishers

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Abstract:

A thermostable DNA photolyase from an extremely thermophilic bacterium, Thermus thermophilus HB8 has been crystallized by the hanging drop vapor diffusion method using NH4H2PO4 as a precipitant. The hexagonal crystals were grown to a size of 0.4 mm in length. X-ray diffraction experiments show the crystals to belong to the hexagonal space group P6 5 22 or P6 1 22 with the unit cell dimensions of a=b=113.6 A, c=142.0 A. They diffract X-rays to 2.4 A resolution with synchrotron radiation.

Keywords: THERMOSTABLE DNA PHOTOLYASE; THERMUS THERMOPHILUS HB8; CPD photolyase; Escherichia coli; Anacystis nidulans

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409157

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