Cdna Cloning And Expression Of Acutin Ii, A Native Mutant Of Thrombin-Like Enzyme From Agkistrodon Acutus

Authors: Pan H.; Liu J.; Du X.; Zhou Y.; Wu X.

Source: Protein and Peptide Letters, Volume 8, Number 6, December 2001 , pp. 469-475(7)

Publisher: Bentham Science Publishers

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Abstract:

One cDNA of a thrombin-like enzyme named acutinII was amplified by RT-PCR from the venom gland of the Agkistrodon acutus snake. AcutinII shows high similarity to acutin, the other thrombin-like enzyme from Agkistrodon acutus snake in nucleotide and amino acid sequences except it is shorter by 16 amino acid at the C-terminus. The catalytic residues and disulfide bridges of acutinII were deduced. AcutinII was expressed in E. coli .The expressed protein was detected by western blotting and the yield was up to 32 percent of the total proteins.

Keywords: CDNA CLONING; ACUTIN II; AGKISTRODON ACUTUS; Snake venom; Thrombin-like enzyme; RT-PCR; Expression; thrombosis

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866013409139

Publication date: 2001-12-01

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Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.