Detection and Purification of Insecticidal Peptides from Scorpions and Spiders: A Rapid Method for Their Isolation from Their Crude Venoms Using MALDI-TOF-MS Analysis

Authors: Gerardo Corzo¹; Elba Villegas¹; Hon-Seok Lee¹; Terumi Nakajima¹

Source: Protein and Peptide Letters, Volume 8, Number 5, October 2001 , pp. 375-383(9)

Publisher: Bentham Science Publishers

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Abstract:

The insecticidal neurotoxins AaIT, LqhIT2, and miew-agaIV from venom of Arachnida were rapidly re-purified from their respective crude venoms using mass spectrometry analyses and two high pressure chromatographic steps. The purity of each toxin was confirmed by capillary zone electrophoresis. Each toxin was identified by mass spectrometry and their biological activity confirmed by bioassays against tobacco cutworms and mice. In addition, AaIT and LqhIT2 were partially sequenced using post-source decay mass spectrometry analysis. The spider neurotoxin miew-agaIV was identified from other isomassic peptide by fingerprint mass analysis of its tryptic fragments.

Keywords: INSECTICIDAL PEPTIDES; MALDI-TOF-MS; insecticidal neurotoxins AaIT; LqhIT; mass spectrometry; capillary zone electrophoresis; Androctonus australis; Leiurus quinquestriatus; Agelenopsis aperta

Document Type: Review article

DOI: 10.2174/0929866013409238

Affiliations: 1: 1-Suntory Institute for Bioorganic Research, Mishima-Gun, Shimamoto-Cho, Wakayamadai 1-1-1, Osaka 618-8503, Japan

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