Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA
Abstract:Two major tRNA binding proteins were obtained by biochemical method from yeast crude extracts of total membrane proteins. They were further confirmed to be tRNA specific-binding proteins by gel mobility shift assay. N-terminal sequence assays showed that they were glyceraldehyde-3-phosphate dehydranase (GAPDH) and 3-phosphate glycerate kinase (PGK1), respectively.
Document Type: Review Article
Affiliations: State Key Laboratory of Molecular Biology, the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China
Publication date: 2001-10-01
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