Isolation and Identification of Two Membrane Proteins Binding to Transfer RNA

Authors: Quan-Sheng Liu1; Jian-Hua Liu1; De-Sheng Jiang1; You-Xin Jin1; De-Bao Wang1

Source: Protein and Peptide Letters, Volume 8, Number 5, October 2001 , pp. 367-374(8)

Publisher: Bentham Science Publishers

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Abstract:

Two major tRNA binding proteins were obtained by biochemical method from yeast crude extracts of total membrane proteins. They were further confirmed to be tRNA specific-binding proteins by gel mobility shift assay. N-terminal sequence assays showed that they were glyceraldehyde-3-phosphate dehydranase (GAPDH) and 3-phosphate glycerate kinase (PGK1), respectively.

Keywords: glyceraldehyde-3-phosphate dehydranase (GAPDH); RNA polymerase III

Document Type: Review article

DOI: 10.2174/0929866013409265

Affiliations: 1: State Key Laboratory of Molecular Biology, the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai 200031, China

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