Hydroxyapatite Elution Behavior of Human Nucleotide Excision Repair Protein XPA and Fragments of XPA

Authors: Garry W. Buchko¹; Michael A. Kennedy¹

Source: Protein and Peptide Letters, Volume 8, Number 5, October 2001 , pp. 357-365(9)

Publisher: Bentham Science Publishers

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Abstract:

The hydroxyapatite elution behavior of 3 fragments of human XPA (M98-F219 = XPA-MBD M59-F219 = XPA-EM M59-M273 = XPA-deltaN58) and full-length human XPA containing a 6-residue, N-terminal histidine tag (h6-XPA) are described. With increasing concentration of potassium phosphate the proteins elute in the order: XPA-EM, XPA-MBD, XPA-deltaN58, and h6-XPA. If hydroxyapatite affinity is related to DNA-binding affinity, then h6-XPA and XPA-deltaN58 may bind DNA more tightly than XPA-MBD and XPA-EM.

Keywords: REPAIR PROTEIN XPA; Nucleotide excision repair (NER); XPA gene

Document Type: Review article

DOI: 10.2174/0929866013409300

Affiliations: 1: Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory, Richland, WA 99352.

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