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Hydroxyapatite Elution Behavior of Human Nucleotide Excision Repair Protein XPA and Fragments of XPA

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The hydroxyapatite elution behavior of 3 fragments of human XPA (M98-F219 = XPA-MBD M59-F219 = XPA-EM M59-M273 = XPA-deltaN58) and full-length human XPA containing a 6-residue, N-terminal histidine tag (h6-XPA) are described. With increasing concentration of potassium phosphate the proteins elute in the order: XPA-EM, XPA-MBD, XPA-deltaN58, and h6-XPA. If hydroxyapatite affinity is related to DNA-binding affinity, then h6-XPA and XPA-deltaN58 may bind DNA more tightly than XPA-MBD and XPA-EM.

Keywords: Nucleotide excision repair (NER); REPAIR PROTEIN XPA; XPA gene

Document Type: Review Article


Affiliations: Environmental Molecular Sciences Laboratory Pacific Northwest National Laboratory, Richland, WA 99352.

Publication date: October 1, 2001

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  • Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.

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