Nucleolin Binds to a cis-Active Region in the Human Desmocollin 2 Promoter
Abstract:Affinity chromatography using oligo DNA from a cis-active region (-453 / -389) of the human DSC2 promoter was used to isolate a protein from human CaCo-2 epithelial cells expressing DSC2. The protein was SDS-PAGE purified, trypsinized and analyzed by MALDI-TOF mass spectrometry. Peptide mass fingerprinting identified the protein as nucleolin, a ubiquitously expressed protein involved in gene regulation
Document Type: Review Article
Affiliations: Division of Membrane Biology1, Division of Protein Structure2, National Institute for Medical Research, Mill Hill, London NW7 1AA, United Kingdom
Publication date: October 1, 2001
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- Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.