Atomic Force Microscopy Investigation of Ribonuclease A
Ribonuclease A (RNase A) molecules have been adsorbed onto mica surfaces from aqueous solution at pH 7.4. Atomic force microscopy (AFM) images of native RNase A show aggregates of monomers each having a diameter of 9 nm. This is consistent with a globular unit the size of which would be substantially larger than that expected for a 13-kDa protein. These experiment suggest that the RNase A oligomer subunits exist as a multimeric complex with the 13-kDa protein.
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Document Type: Review Article
Affiliations: Departamento de Genetica e Morfologia, UnB, Brasilia, DF, Brazil.
Publication date: 2001-10-01
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