Crystallization and Preliminary X-Ray Crystallographic Studies of Human Autocrine Motility Factor

Authors: Uemura H.; Tanaka N.; Funasaka T.; Haga A.; Nagase H.; Raz A.; Nakamura K.T.

Source: Protein and Peptide Letters, Volume 8, Number 4, August 2001 , pp. 317-322(6)

Publisher: Bentham Science Publishers

Key:

- Free Content

- New Content

- Subscribed Content

- Free Trial Content

Abstract:

Human autocrine motility factor (hAMF), a tumor-secreted cytokine which stimulates cell migration in vitro and metastasis in vivo, has been crystallized by the hanging-drop vapour diffusion method. The crystals belong to an orthorhombic space group P212121 with the cell dimensions of a = 80.79 A, b = 107.1 A, and c = 270.9 A. There are two dimers per asymmetric unit. The crystals diffract to at least 2.0 A resolution and are suitable for X-ray structure analysis at high resolution.

Keywords: Crystallization; Motility Factor; cytokine

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409364

The full text electronic article is available for purchase. You will be able to download the full text electronic article after payment.

$55.10 plus tax Refund Policy