One-Step Affinity Purification of Human Mu-Opioid Receptor Overexpressed in Baculovirus System

Authors: Wei C.Li.; Ping F.Ya.; He Z.De; xue-Jun X.; Jie C.; Qiang W.; Zhi-Qiang C.

Source: Protein and Peptide Letters, Volume 8, Number 4, August 2001 , pp. 265-272(8)

Publisher: Bentham Science Publishers

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Abstract:

Affinity chromatography on Ni-nitrilotriacetic acid agarose was used to purify human mu-opioid receptors overexpressed in Sf9 cells. We introduced a tag of 6 consecutive histidines at its carboxyl terminus for easy purification of recombinant proteins. The binding activity and identification of the purified receptor were determined by receptor binding assay, SDS-PAGE and Western blotting analysis. This procedure used in the paper offer an easy and fast route to the purification of recombinant human mu-opioid receptors.

Keywords: BACULOVIRUS; opioid receptor (MOR); Spodoptera frugiperda; Trichoplusia ni; Receptor binding assay

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409373

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