Effect of Lyotropic Salts on the Stability of a Subtilisin-Like Proteinase from a Psychrotrophic Vibrio-Species, Proteinase K and Aqualysin I.

Authors: Kristjansson M.M.; Magnusson O.Th.

Source: Protein and Peptide Letters, Volume 8, Number 4, August 2001 , pp. 249-255(7)

Publisher: Bentham Science Publishers

Abstract:

The stability of three related subtilisin -like proteinases a proteinase from a psychrotrophic Vibrio-species, proteinase K and aqualysin I from the thermophile Thermus aquaticus, to denaturation by GdmSCN, was measured in the presence and absence of selected lyotropic salts (sulfate and thiocyanate). How these salts affect protein stability has been ascribed to their effect on the strength of hydrophobic interactions. The salts affected the stability of the proteinases according to their ranking in the lyotropic salt series. The effect of the salts was smallest, however, on the stability of the cold-adapted Vibrio-proteinase, but largest for the thermophilic aqualysin I. These results suggests that the cold-adapted enzyme may be less dependent on hydrophobic interactions for structural stability than its counterparts adapted to higher temperatures.

Keywords: LYOTROPIC SALTS; SUBTILISIN-LIKE PROTEINASE; VIBRIO-SPECIES; PROTEINASE K; AQUALYSIN; subtilisin-like proteinases; proteinase K; Aqualysin; Aqualysin; Thermus aquaticus; proteinases

Language: English

Document Type: Review article

DOI: http://dx.doi.org/10.2174/0929866013409355

Publication date: 2001-08-01

• Protein & Peptide Letters publishes short papers in all important aspects of protein and peptide research, including structural studies, recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, drug design etc. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallisation, and preliminary structure determinations of biologically important proteins are acceptable. Purely theoretical papers are also acceptable provided they provide new insight into the principles of protein/peptide structure and function.

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• By this author: Kristjansson M.M. ;  Magnusson O.Th.

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