Crystallizationand Preliminary X-Ray Crystallographic Studies of Formaldehyde Dehydrogenase from Pseudomonas putida

Authors: Kusakabe Y.; Tanaka N.; Ito K.; Yoshimoto T.; Nakamura K.T.

Source: Protein and Peptide Letters, Volume 8, Number 3, June 2001 , pp. 237-240(4)

Publisher: Bentham Science Publishers

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Abstract:

The formaldehyde dehydrogenease from Pseudomonas putida (PFDH), has been crystallized by the vapour diffusion method using ammonium sulfate as the Precipitating agent. The crystals belong to a trigonal space group P3 12 (or P3 12) with the cell dimensions of a = b = 85.74 A, and c = 190.9 A. There are two subunits per asymmetric unit. The crystals diffract to at least 2.2 A resolution using Cu K Alfa radiation at 100 K. Self-rotation function studies suggest that the tetrameric PFDH molecular has the 222 point group symmetry.

Keywords: Formaldehyde Dehydrogenase; Pseudomonas Putida; PFDH molecule; glutathione-dependent FDHs

Language: English

Document Type: Review article

DOI: 10.2174/0929866013409454

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